Mesophilic amylase Cas 9000-90-2

Catalog of Enzyme Preparations
20240409

                                                                                  Mesophilic amylase
【Product name】Mesophilic amylase
【Definition of enzyme activity】 The amount of enzyme that liquefies 1g of soluble starch in 1 hour under the conditions of 60°C and PH 6.0 is one enzyme activity unit (U).
【Product specifications】 5,000 ~ 200,000 u/g, different energy products can be produced according to customer needs.
【Conditions of Use】
Effective range: ph6.0-9.0, optimal function ph6.0-6.5;
The most suitable temperature is 60℃-80℃. Above 70℃, as the temperature increases, the reaction speed accelerates, but the deactivation also accelerates.
【CAS No.】9000-90-2

1.Product specifications: 

2. Product features:

Mesophilic amylase has the characteristics of high enzyme activity, good heat resistance, and wide pH range. It can maintain a high reaction rate within a certain temperature range (usually 60°C ~ 80°C), but too high a temperature can also cause its deactivation. In addition, mesophilic amylase also has certain requirements for pH value. The optimal pH value is usually between 6.0 and 6.5. A pH value that is too low or too high will affect its activity.

3. Production process:

The production of medium-temperature amylase mainly goes through microbial fermentation, extraction, refining and other steps. First, fermentation is carried out by specific microorganisms (such as Bacillus subtilis), then the enzymes in the fermentation broth are extracted, and finally the finished product is refined. The entire production process requires strict control of fermentation conditions, extraction processes and refining methods to ensure product quality and activity.

4. Process characteristics:

The production process of medium-temperature amylase has the advantages of simple operation, low cost, and easy control. At the same time, due to the use of advanced extraction and refining technology, the purity and activity of the product are also effectively guaranteed.

5. Product application:

Medium-temperature amylase is widely used in many fields. The following is an overview of its application in the fields of medicine, health care, cosmetics, food, chemical industry, feed and agriculture:

Pharmaceutical field:
In the pharmaceutical field, medium-temperature amylase is mainly used as pharmaceutical excipients or for starch hydrolysis in the pharmaceutical process. By breaking down starch, it can improve the solubility, stability and bioavailability of drugs, thereby increasing their efficacy.

Health care field:
In the field of health care, the application of medium-temperature amylase is mainly to help the human body better digest and absorb the starch components in food, thereby improving the utilization of nutrients. This has a positive impact on maintaining intestinal health and improving digestive function.

Cosmetics field:
Although medium-temperature amylase has relatively few applications in the field of cosmetics, it may still be used as a raw material for the preparation of certain cosmetic ingredients. For example, it can be used to improve the texture and stability of cosmetics and enhance the experience of using the product.

Food field:
In the food industry, medium-temperature amylase is particularly widely used. It can be used in the production of bread, beer, maltose, MSG and other products to improve the taste, color and texture of food by decomposing starch. In addition, medium-temperature amylase can also be used to preserve and preserve food, extending the shelf life of food.

Chemical industry:
In the field of chemical industry, medium-temperature amylase can be used as a biocatalyst for certain chemical reactions to increase reaction speed and efficiency. This helps reduce energy consumption and pollution in the chemical production process and achieve green production.

Feed field:
In the feed industry, mid-temperature amylase is mainly used to improve the digestibility and nutritional value of feed. By decomposing starch in feed, it can improve feed utilization and promote animal growth and development. In addition, mid-temperature amylase can also prevent diarrhea and regulate intestinal microorganisms, helping to maintain the health of animals.

Agriculture:
In the agricultural field, medium-temperature amylase can be used in the planting and processing of crops. For example, it can be used to improve the growing environment of crops and increase the yield and quality of crops. At the same time, medium-temperature amylase can also be used in the processing of agricultural products, such as the extraction and modification of starch.

It should be noted that although medium-temperature amylase is used in many fields, its specific use needs to be selected and adjusted according to the characteristics and requirements of different fields.

6. Recommended dosage: 

1. During the beer brewing process, add the amylase after mixing the slurry in the gelatinization pot. The amount of enzyme added is about 6 units/gram of raw material, and the amylase is liquefied at 85-90°C for about 30 minutes.
2. In the production of maltose and enzymatic MSG, add amylase to the prepared starch slurry (about 6-8 units/gram of raw material), mix thoroughly, and then heat to 85-90°C to liquefy for about 30 minutes.
3. Application in textile desizing, suitable for the desizing process of silk, chemical fiber, cotton and wool fabrics that are not resistant to high temperatures. The amount of enzyme added is about 0.2% (2000u/ml), and the water temperature is maintained at 50-80℃ for 20-20%. 40 minutes.
4. In other industries, the amount of enzyme is generally controlled to be 6-8 units/gram of starch, and the calcium ion concentration is 150ppm.

7. Precautions

1. Mesophilic amylase is a biologically active substance that is inhibited and damaged by heavy metal ions (Fe3+, Cu2+, Hg+, Pb+, etc.) and oxidants, and contact with them should be avoided.

2. It is recommended to wear protective equipment when using enzyme products; if enzyme powder or enzyme solution accidentally comes into contact with the eyes, causing tears and corneal redness, rinse with clean water first, then instill eye drops, and you will recover the next day. Allergy, itching, redness and swelling caused by contact with the face, eyelids and skin can be cured by applying Pikan Cream or Sanjiu Dermatitis Ping.

3. There may be color differences between different batches of products.

8. Product storage:

Moisture-proof, light-proof, sealed, stored at low temperature (0-10℃), shelf life is 24 months. There is a certain loss of enzyme activity during storage. The enzyme activity loss is 5-10%/6 months when stored at 10°C, and the enzyme activity loss is 10-15%/6 months when stored at room temperature.

9. Packaging, transaction method, delivery method, etc.:

Packaging: 1kg/25kg bag/drum with linner inside.

Payment method: Western Union; T/T; MoneyGram

Shipping method: Sea/Air/Domestic Shipping}

10. After-sales service/customized service/sample service:

Packaging customization: Customized services are provided for packages above 1000KG;

Sample service: samples are available;